The OhmX Sample Prep workflow provides a seamless path from DNA to valuable insights on the OhmX Analyzer. Our workflow is amplification-free for unbiased, whole genome-wide structural variant detection. This user-friendly workflow allows you to go from cells to injection in just one day using standard molecular biology techniques, without the need for additional equipment.
DNA Isolation
Sample preparation
Easy-to-use workflow
Expedite your research and detect a broadened range of structural variants with electronic genome mapping starting with an easy to follow sample preparation workflow.
Our recommended High-Molecular Weight (HMW) DNA extraction workflow is NEB’s Monarch(r) HMW DNA Extraction Kit for Cells & Blood #T3050S. This protocol has been further optimized for use with the OhmX platform, which can map long-range HMW DNA molecules of 50kb-500kb. This streamlined ~60 minute protocol yields more than 5 μg of pure high-molecular weight DNA with flexible input requirements of only ~1 million cells or 200-500uL of blood.
Our DNA labeling process involves precisely tagging high-molecular weight DNA at known recognition sites using sequence-specific nicking enzymes. On average, the interval spacing between the tagged sites is ~4kb per HMW DNA molecule allowing for high-density tagging across the whole genome. This process creates tagged DNA molecules that are then electronically detected as they traverse channels on the OhmX Analyzer. The resulting data provides a comprehensive and precise genome-wide map of tagged positions, offering insights into structural variations, rearrangements, and genomic alterations with unparalleled precision and efficiency.
OhmX Sample Prep Kit: The sample preparation process entails sequence-specific DNA nicking, precise labeling and tagging of these nicked sites with a proprietary tag to enhance electronic detection. The final step of sample preparation involves coating the DNA with a DNA binding protein to increase the signal in the detector and ensure the precise detection of sequence-specific tags.
60 Clifford Street
Providence, Rhode Island
02903 USA
Phone: 401.276.9100
Fax: 401.276.9122
info@nabsys.com
Advance the understanding of disease, increase diagnostic yield, and improve patient outcomes by enabling routine, accurate, cost-effective analysis of genomic structural variation
